英语翻译III.A protein of unknown structure has been purified and

英语翻译
III.A protein of unknown structure has been purified and is subjected to gel filtration chromatography under different conditions,with the following results:1) Chromatography under native conditions suggests a molecular weight of 240,000 from comparison to standards of known molecular weight chromatographed on the same column; 2) Chromatography in the presence of 5 M urea yields a single protein peak corresponding to a M.W.of 60,000 from comparison to standards of known molecular weight chromatographed on the same column in urea.
1.What information do the results of (1) and (2) give about the structure of the protein?Describe the structure as completely as the data permit;
2.On an SDS polyacrylamide gel,the pure protein shows two stained bands.The relative mobilities of those 2 "unknown" bands are shown on the plot below as open circles (O),with the mobilities of 5 known polypeptide standards of M.W.15,000; 20,000; 30,000,40,000 and 50,000.Given the results of the SDS gel,how would you alter your description of the protein structure from the description you gave in part Be as specific as you can about your revised description of the structure,and explain the reason for the altered description.
III．You are working on a compound as a potential drug that inhibits an overproduced enzyme whose uncontrolled activity leads to uncontrolled cell proliferation (cancer).A steady-state kinetic analysis of the enzyme-catalyzed reaction was carried out,with velocities measured as a function of the concentration of the substrate in the absence and in the presence of 10 nM inhibitor.The results of the kinetics experiment are plotted for you below.Please answer the following questions.(10 pts)
What type of inhibitor is this compound (be as specific as you can)?Show the diagram of the inhibitor’s mechanism (by E denoting enzyme,S denoting substrate,I denoting inhibitor; for example:).
Calculate the Km,Kcat in the absence of inhibitor and Kmapp,Kcatapp in the presence of inhibitor (app denoting apparent.The total concentration of enzyme is 2nM).
3.Calculate the inhibitor constant KI.
A reaction system includes substance A,substance B and an enzyme.The figure at right shows the Lineweaver-Burk Plot for the enzyme.If substance A acts as a substrate of the enzyme,referring to the figure,please do:(1) describing the possible role of substance B in the reaction system; (2) comparing the magnitude of the concentration [B]1,[B]2,and [B]3; (3) the scheme of the kinetic mechanism of the reaction system
前两位一看就知道你们使用翻译软件翻得，看看语句就知道了，毫不通顺。要是那么容易我又何必提问还悬赏那么多分呢！

第三次世界大战.一种蛋白质的结构已经洁净了,未知受凝胶过滤层析条件下的试验,结果表明:1)色谱条件下提出了一个土生土长的分子量240,000从比较标准的分子量在同一列柱层析;(2)在5米尿素产量高峰期单一蛋白分子量的对应的标准从比较6已知的分子量时在同一列在尿素.
1.什么信息”的结果,(1)和(2)提供有关蛋白结构的吗?描述了数据结构完整;
2.在一个标准的聚丙烯酰胺凝胶、纯蛋白质显示两个染色乐队.对那些相对机动性2“未知”乐队的阴谋下开放的圆(11),与机动性的分子量多肽标准的5个已知15000;2、3、4、5万人.给出了SDS凝胶,你会怎样改变你所描述的蛋白质结构的描述你给在第1部分?作为具体你可以对你的修订描述的结构,并解释原因.改变
III.You正在研发一种化合物作为潜在药物抑制酶的活性的overproduced失控,导致细胞增殖失控(癌症).稳态酶催化反应的动力学分析进行了测量,速度的浓度的基体未及10海里乙酰胆碱酯酶抑制剂.动力学实验的结果下面为你绘出了.请回答以下问题.(10分).
什么类型的乙酰胆碱酯酶抑制剂是该化合物(如具体你可以吗?图展示的乙酰胆碱酯酶抑制剂的机制(E - S -酵素、基质,我表示乙酰胆碱酯酶抑制剂,例如:).
计算公里,Kcat在缺乏乙酰胆碱酯酶抑制剂和Kmapp,Kcatapp的乙酰胆碱酯酶抑制剂(app指示明显.总浓度的酶是2nM).
3.计算的乙酰胆碱酯酶抑制剂常数的吻.
一个反应系统包括物质,物质和酶.在正确的数字显示Lineweaver-Burk阴谋的酶.如果物质是充当一个基片的酶,参照图,请务必:(1)描述的可能作用的物质,在反应系统;(二)比较集中的大小(B)1、2、3期(B);(3)方案的反应系统动力学机制